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A, Western immunoblot of a NEIEF gel, stained with TnI antibody of failing hearts. B, frequency dependency of TnI in failing hearts but not in control. Abbreviations used: TnI, troponin I; U, unphosphorylated; P*, monophosphorylated; and P**, biphosphorylated. Values are expressed as means ±s.e.m. (Control: 0 Hz (n= 4); 3, 6 and 9 Hz (n= 5); Failing: 3 Hz (n= 5), 0, 6 and 9 Hz (n= 6).
