Quantitative analyses for RyR subtypes were performed by real-time PCR. RyR1 (A), RyR2 (B) and RyR3 (C) mRNA expression levels were compared between RyR2+/+ and RyR2+/−. A, n= 4, except for portal vein and aorta of RyR2+/− (n= 3); B, n= 4, except for urinary bladder of RyR2+/+ (n= 8) and portal vein and aorta of RyR2+/− (n= 3); C, n= 4, except for portal vein and aorta of RyR2+/− (n= 3). D, comparison of mRNA expression levels of RyR1–3 in urinary bladder. n= 4, except for RyR2 of RyR2+/+. *P < 0.05 versus RyR2+/+.