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. 2006 Dec 7;579(Pt 1):269–284. doi: 10.1113/jphysiol.2006.121079

Figure 8. Representative gels of GLUT4 protein and insulin signalling intermediates of longissimus dorsi muscle for the oil treatment sequence of the switchback design ‘control–LCn–3PUFA–control’.

Figure 8

A, phosphorylation state with protein amount of PKB on its Ser473 residue (P = 0.03); B, phosphorylation state with protein amount of mTOR on its Ser2448 residue (P = 0.06), S6K1 on its Thr389 residue (P = 0.04), and 4E-BP1 on its Ser65 residue (P = 0.10), respectively; C, GLUT4 protein abundance (P = 0.08). This data set combines signalling data from muscle biopsies carried out in experimental periods 1, 2 and 3 of the switchback design (providing n = 9 per treatment in total with n = 8 for the control treatment) with additional signalling data from a 2nd cohort of 6 steers originating from a Latin square design that provides n = 6 per treatment. This yielded n = 13 and 14 observations per treatment for a total of 27 and 28 for control and menhaden oil treatment, respectively. Details are provided in Methods.