Table 5.

Fatty acid composition (% by weight) of longissimus dorsi phospholipid fractions of steers infused for 35 days with control or menhaden oil rich in LCn–3PUFA

	Control oil	Menhaden oil	s.e.m.	P-value
Phosphatidylcholine
14: 0	0.5	0.4	0.2	ns
16: 0	27.3	27.7	0.5	ns
16: 1 n–7	0.4	0.9	0.2	0.04
18: 0	5.6	5.7	0.4	ns
18: 1	10.3	9.3	0.6	ns
18: 2 n–6	39.4	24.9	1.9	< 0.01
18: 3 n–3	0.7	1.2	0.1	< 0.01
20: 3 n–6	1.1	1.4	0.1	0.03
20: 4 n–6	4.2	5.8	0.5	< 0.01
20: 5 n–3	7.6	17.7	1.6	< 0.01
22: 5 n–3	1.7	1.8	0.2	ns
22: 6 n–3	1.2	3.3	0.2	< 0.01
Phosphatidylethanolamine
14: 0	nd	nd
16: 0	3.7	3.6	0.5	ns
16: 1 n–7	nd	nd
18: 0	22.7	24.6	0.8	ns
18: 1	8.6	6.3	0.6	0.01
18: 2 n–6	19.1	11.1	1.7	< 0.01
18: 3 n–3	nd	nd
20: 3 n–6	1.4	0.8	0.3	0.04
20: 4 n–6	14.9	14.1	0.7	ns
20: 5 n–3	19.9	26.1	2.9	0.07
22: 5 n–3	6.2	4.4	0.4	0.02
22: 6 n–3	4.1	8.5	0.6	0.01
Phosphatidylinositol
14: 0	nd	nd
16: 0	4.4	6.6	0.6	0.02
16: 1 n–7	nd	nd
18: 0	47.5	43.4	1.3	0.05
18: 1	9.7	9.9	0.6	ns
18: 2 n–6	11.9	8.5	0.9	0.03
18: 3 n–3	nd	nd
20: 3 n–6	2.9	3.0	0.4	ns
20: 4 n–6	10.2	9.9	0.4	ns
20: 5 n–3	7.7	9.6	1.1	0.09
22: 5 n–3	4.0	5.4	0.4	0.03
22: 6 n–3	1.9	3.6	0.3	< 0.01
Phosphatidylserine
14: 0	nd	nd
16: 0	8.1	5.7	1.0	ns
16: 1 n–7	nd	nd
18: 0	47.3	45.8	1.9	ns
18: 1	15.9	14.3	1.6	ns
18: 2 n–6	17.8	10.1	1.1	< 0.01
18: 3 n–3	nd	nd
20: 3 n–6	1.7	2.1	0.6	ns
20: 4 n–6	3.5	4.1	0.9	ns
20: 5 n–3	1.1	3.7	0.6	0.01
22: 5 n–3	2.7	6.2	0.6	< 0.01
22: 6 n–3	1.9	8.0	0.6	< 0.01

Least square means ±s.e.m.; n = 9 observations per treatment resulting from testing 2 treatments over 3 experimental periods in 6 steers according to a switchback design; nd = not detected; ns, not significant.