Table 2.
Protein recovery, enrichment of plasma membrane marker enzymes and plasma membrane, endosome and lysosome purity in organelles isolated from rats hepatocytes
| SD–Rest | SD–Ex | HF–Rest | HF–Ex | |
|---|---|---|---|---|
| Protein recovery (mg protein g−1) | 1.91 ± 0.20 | 1.84 ± 0.18 | 1.78 ± 0.15 | 1.80 ± 0.24 |
| Relative specific activity | ||||
| Membranes | ||||
| 5′-ND | 13.01 ± 0.65 | 12.68 ± 0.67 | 10.13 ± 0.75 | 9.98 ± 0.59 |
| GGTP | 5.02 ± 1.31 | 4.99 ± 1.5 | 5.60 ± 1.37 | 5.40 ± 1.42 |
| Na,K-ATPase | 16.66 ± 1.41 | 16.45 ± 1.55 | 15.96 ± 0.98 | 14.77 ± 0.99 |
| SDH | 0.18 ± 0.04 | 0.26 ± 0.06 | 0.24 ± 0.07 | 0.27 ± 0.08 |
| G6Pase | 0.83 ± 0.09 | 0.84 ± 0.08 | 0.64 ± 0.12 | 0.67 ± 0.11 |
| Lysosomes | ||||
| 5′-ND | 0.8 ± 0.09 | 1.0 ± 0.12 | 0.9 ± 0.15 | 1.1 ± 0.03 |
| Arylsulphatase | 60 ± 12 | 65 ± 21 | 63 ± 11 | 66 ± 21 |
| N-acetyl-β-glucosaminidase | 55 ± 9 | 59 ± 13 | 54 ± 9 | 53 ± 15 |
| Succinate-INT-reductase | 0.04 ± 0.01 | 0.09 ± 0.01 | 0.07 ± 0.03 | 0.1 ± 0.05 |
| G6Pase | 0.07 ± 0.02 | 0.12 ± 0.02 | 0.09 ± 0.01 | 0.08 ± 0.02 |
| Endosomes | ||||
| 5′-ND | 1.2 ± 0.09 | 1.4 ± 0.07 | 1.1 ± 0.1 | 1.2 ± 0.08 |
| Arylsulphatase | 100 ± 15 | 113 ± 18 | 109 ± 10 | 102 ± 11 |
| N-acetyl-β-glucosaminidase | 98 ± 12 | 103 ± 15 | 96 ± 7 | 103 ± 14 |
| Succinate-INT-reductase | 0.4 ± 0.05 | 0.57 ± 0.1 | 0.41 ± 0.03 | 0.61 ± 0.11 |
| G6Pase | 0.3 ± 0.04 | 0.60 ± 0.07 | 0.52 ± 0.06 | 0.55 ± 0.12 |
Relative specific activity for each enzyme was calculated by dividing its specific activity in plasma membrane by its specific activity in homogenate. SD–Rest: standard diet-rest; SD–Ex: standard diet–acute exercise; HF–Rest: high-fat diet–rest; HF–Ex: high-fat diet–acute exercise; SDH: succinate deshydrogenase; INT: iodonitrotetrazolium violet.