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. 2006 Dec 14;579(Pt 2):413–429. doi: 10.1113/jphysiol.2006.123133

Figure 4. MCD effect on neurotransmission is not dependent on the presence of glial cells.

Figure 4

A and B, hypertonic sucrose stimulation after MCD treatment in the absence of glia. A, sample traces. B, summary graph showing that the average charge transfer during the first 10 s of the 30 s sucrose response is decreased 94% after treatment with MCD compared with cultures treated with 20 mm K+ alone. Horizontal bar represents the presence of hypertonic sucrose; 1 culture, 20 mm K+ alone, n = 6; 20 mm K+ with MCD, n = 6. C and D, field stimulation evoked responses after MCD treatment in the absence of glia. C, sample traces. D, summary graph showing an 85% reduction in the average evoked EPSC amplitude for cultures treated with MCD compared with cultures treated with 20 mm K+ alone. Arrow represents timing of the stimulation; 1 culture, 20 mm K+ alone, n = 6; 20 mm K+ with MCD, n = 3. E–G, mEPSCs after MCD treatment in the absence of glia. E, sample traces. F, summary graph shows a 3-fold increase in the frequency of mEPSCs for cultures treated with MCD compared with cultures treated with 20 mm K+ alone. G, the distributions of mEPSC amplitudes were not affected by these treatments as determined by the K-S test (P > 0.001). 1 culture; 20 mm K+ alone, n = 6; 20 mm K+ with MCD, n = 9. Error bars represent the s.e.m.*P < 0.05, **P < 0.005.