Table 1.

Effect of neonatal maternal deprivation on colonic preproCRF immunohistochemistry in adult rats

	Control	NMD
Sub mucosa	0.6 ± 0.2	0.9 ± 0.2
Lamina propria	0.2 ± 0.2	1.1 ± 0.3*
Enterocytes	1.1 ± 0.3	2.4 ± 0.4*
Colonic bottom crypts	0.6 ± 0.4	1.5 ± 0.2*
Total CRF	2.5 ± 0.6	5.9 ± 0.7*

Results were obtained from at least six different rats from control and NMD groups. Data (mean ± s.e.m.) are expressed as the total scoring, graded from 0 to 3 (0, no immunoreactivity; 1, weak immunoreactivity; 2, intermediate immunoreactivity; 3, strong immunoreactivity).

^*P < 0.05 from control (Student's t test).