Trisk95 was immunoprecipitated from skeletal muscle extracts and immunoprecipitates were immunoblotted for phospho-Thr (top panel) and trisk95 (middle panel). The bottom panel is signal intensity for phospho-Thr relative to trisk95 expression. Representative immunoblots are shown; C, non-stimulated contralateral muscle: STIM, electrically stimulated to contract: CON, contralateral muscle. Data are mean ± s.e.m., n = 6; *different from time 0, P < 0.001.