Figure 3. T5KO-H mice have increased permeability to macromolecules.

T5KO-L mice (noncolitic), T5KO-H mice (colitic), and WT littermates were fasted for 3 hours and then given FITC-dextran (4 kDa or 40 kDa) and HRP intragastrically as described in Methods. Three hours later, mice were bled, and serum concentrations of FITC and HRP were determined by fluorimetry and spectroscopy. *P < 0.05, significant difference from WT mice.