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Immunochemical analysis of plasma membrane vesicle- and secretory vesicle-enriched fractions for other cell organelles. An equal number of cell equivalents of plasma membrane vesicles (PMV), and secretory vesicles SV, were separated by SDS-PAGE, electroblotted, and probed with antibodies against calreticulin and calnexin (both molecular chaperones residing in the ER), golgin 97 (marker for Golgi), porin and cytochrome c (both markers of mitochondria).
