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Evaluation of three transduction protocols. A) Effect of PB at the indicated concentrations on LA34/VSV-G transduction of 293T cells. B) Effect of using the standard (empty columns), PB (solid columns) or PB/DT protocol (striped columns) on LA34/VSV-G transduction of the indicated cells. Bars and percent GFP-positive cells evaluated as in Fig. 4. ND, not done.
