Table 1.

Vector titers generated from transfected 293T or CrFK cells^a

Vector/Env used for pseudotyping	Vector produced in	Vector titer (RNA copies/ml)	Concentration	Transduction units/mlb
LA34/VSV-G	CrFK	8.5 × 109	None	3.8 × 106
	293T	1.8 × 109	None	1.2 × 106
LAW34/VSV-G	293T	5.0 × 109,c	None	2.5 × 107
		2.1 × 1010,c	Ultracentrifugation	3.0 × 107
	293T	7.0 × 109	PBd	3.0 × 107
LAW34/RD114/TR	293T	1.4 × 1010,c	Ultracentrifugation	6.0 × 107
		4.0 × 109,c	PB	1.2 × 108

^a Titers measured in supernatants collected 2 days post-transfection, average of three independent experiments.

^b Measured in 293T cells.

^c Same supernatant, titrated as such or after 10 fold concentration by ultracentrifugation.

^d Polybrene.