Table 2.

LA34/VSV-G pseudotyped particles generated 2 days post-transfection in 293T cells^a

Packaging construct used for vector production	p25 optical density	Vector RNA copies/ml	Transduction units/mlb
pΔenv1	2.24	9.3 × 109	6.4 × 106
pΔenv2	>2.50	3.0 × 109	3.4 × 106
pGP-CTE	1.09	2 × 106	1.0 × 103
pGP-RREc	1.92	8.1 × 108	3.0 × 105

^a Average of three independent experiments, mean efficiency of transfection 80% (range 75–90%);

^b Titer measured in the indicated cells 2 days post-transduction;

^c Rev provided in trans by cotransfection of pcDNA-Rev.