Fig. 1.

SDS-PAGE analysis of purified reqIgGs. ReqIgGs were analysed by SDS-PAGE and stained with Coomassie under (A) non-reducing conditions and (B) reducing conditions. (C) Immunoblot analysis under non-reducing conditions probed with rabbit anti-mouse λ L chain cross out (C). sIgG, horse serum IgG; huIgG, recombinant human IgG1 containing mouse λ L chains. On each gel, an equivalent quantity of each reqIgG subclass was loaded (5 μg for Coomassie stained gels and 1 μg for immunoblots). The “doublet” bands apparent in some subclasses under non-reducing conditions probably reflect heterogeneity in glycosylation.