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. 2007 Feb 1;580(Pt 2):485–496. doi: 10.1113/jphysiol.2006.124602

Figure 5. Bilateral microinjection of 15 μM MUS in the preBötC.

Figure 5

Schematic representation of the slice preparation showing microinjection pipettes. Below each photograph of microinjection pipettes the XII trace shows respiratory motor output in vitro and the ‘microinject’ trace reflects transistor–transistor logic (TTL) pulses at 3 Hz that gate the micropressure drug-delivery injections. Bilateral microinjection of MUS stops the XII rhythm within 1 min, whereas microinjection of MUS unilaterally in the preBötC does not cause rhythm cessation; microinjection pipettes were moved one at a time 90–150 μm. All traces depict the last 40 s of data acquired in each pharmacological condition. Circles mark the effective location for microinjection pipettes. Time calibration is shown.