Figure 6. Rat jejunal mucosa expresses β3 mRNA and β3 protein is located in the apical membrane.
A, RT-PCR was performed for the four known auxiliary Cavβ subunits using primers designed to rat specific sequences and cDNA derived from rat brain and rat jejunal mucosa. B, jejunum was perfused for 30 min with 75 mm glucose; brush-border membrane (BBM) and basolateral membrane (BLM) vesicles were then prepared and Western blotted for β3. The BBM band was eliminated by neutralization of antibody with excess peptide (data not shown).