FIG. 7.

SRC-3 participates in regulating cell size. PC3 cells transfected with siRNAs were analyzed by immunoblotting for indicated proteins, and cell size were determined using flow cytometry. PC3 cells transfected with siRNA were grown under conditions as described in Materials and Methods. (A) Immunoblot analyses show that SRC-3 siRNAs specifically down-regulate their SRC-3 gene expression. Note that in cells transfected with SRC-3 siRNA, AKT and phospo-AKT are also decreased. (B) The mean FSC-H values of mock-transfected cells (only in the presence of lipofectamine 2000) and of cells transfected with the indicated siRNAs. Luc, luciferase siRNA; SRC-3, SRC-3 siRNA. The means of cell sizes (FSC-H) ± standard deviation (n = 5) are shown.