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. 2003 Nov;23(21):7689–7697. doi: 10.1128/MCB.23.21.7689-7697.2003

FIG. 2.

FIG. 2.

Anaphase segregation assay. Anaphase nuclei were stained blue with DAPI and hybridized with FISH probes to detect artificial and natural chromosomes. In each example, the green alpha satellite FISH probe hybridized to the artificial chromosome as well as natural chromosomes with the same alpha satellite DNA as the artificial chromosome. A red vector probe hybridized to the artificial chromosomes, but not the natural chromosomes. Thus, the natural chromosomes appear as green dots, while the artificial chromosomes appear asmerged green and red signals. (A) Cell line X-5. The natural X chromosomes segregated 2:2, and the artificial chromosomes segregated 1:1. (B) Cell line 17-2. The natural chromosomes 17 segregated 4:4, and the artificial chromosomes segregated 2:4, reflecting a likely nondisjunction event. (C) Cell line X-5. The natural X chromosomes segregated 2:2, and the artificial chromosomes segregated 1:2 with a lagging artificial chromosome in between the other segregating chromosomes. (D) Cell line 17-3. The natural chromosomes 17 segregated 4:4, and one of the artificial chromosomes was in an anaphase bridge.