FIG. 3.

evl-14 and scc-3 encode cohesion proteins. (A and B) Phylogenetic trees of PDS-5 and SCC-3 family members in different species. The trees were computed using MacVector 6.5.3 software. Ce, C. elegans; Hs, Homo sapiens; An, Aspergillus nidulans; Sm, Sordaria macrospora; Sp, Schizosaccharomyces pombe; Sc, S. cerevisiae; Dm, Drosophila melanogaster. The overall degrees of relatedness between the C. elegans proteins and those in other species are indicated in parentheses (percent identity/percent similarity). (C) evl-14 and scc-3 gene structures. Gene structures were determined on the basis of assessment of WORMBASE and cDNA clones. For evl-14, there are no existing cDNA clones that cover the N-terminal region (exons 1 to 8). We have performed RT-PCR analysis of the gene and obtained cDNA sequences from exons 2 to 8. The locations and natures of the molecular lesions of three alleles are indicated.