TABLE 1.
Gene and RNAi method | Genotypea | % of study population exhibiting indicated phenotype
|
n | ||||
---|---|---|---|---|---|---|---|
Embryo lethality | Larva lethalb | Pvl-Stec | Himd | WTe | |||
scc-3 | |||||||
Injection | WT | 100 | 0 | 0 | 0 | 0 | >100 |
Feedingf | WT | NAg | 23.1 | 76.9 | 0 | 0 | 217 |
evl-14 | |||||||
Feeding | WT | 3.7 | 2.8 | 83.5 | 0.9 | 9.2 | 218 |
evl-14(ar96)/ evl-14(+) | 7.2 | 3.9 | 86.5 | 2.4 | 0 | 207 | |
Injection | WT | 13.8 | 4.6 | 79.4 | 2.3 | 0 | 218 |
evl-14(ar96)/ evl-14(+) | 20.5 | 7.9 | 66.9 | 4.6 | 0 | 239 |
Genotype of the worm strain being treated with RNAi.
Animals arrested at various larval stages.
Protruding vulva coupled with sterility phenotype.
High incidence of male (Him) phenotype; males resulted from self-fertilization of hermaphrodites.
Hermaphrodites with normal morphology. WT, wild type.
Embryos were put on RNAi feeding plates for examination of postembryonic phenotypes.
NA, not applicable.