TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| YCplac33 | ARS1 CEN4 URA3 | 26 |
| YEplac195 | 2μm URA3 | 26 |
| YIplac211 | URA3 | 26 |
| YCplac111 | ARS1 CEN4 LEU2 | 26 |
| YEplac181 | 2μm LEU2 | 26 |
| pRS313 | ARSH4 CEN6 HIS3 | 26 |
| pTAT2c | 2.6-kb fragment containing TAT2 promoter-TAT2 in YCplac33 | 3 |
| pTAT1e | 2.6-kb fragment containing TAT1 promoter-TAT1 in YEplac195 | This study |
| pTAT2e | 2.6-kb fragment containing TAT2 promoter-TAT2 in YEplac195 | 3 |
| p3HA-TAT1c | 2.7-kb fragment containing TAT2 promoter-3HA-TAT1 in YCplac33 | This study |
| p3HA-TAT2c | 2.7-kb fragment containing TAT1 promoter-3HA-TAT2 in YCplac33 | This study |
| p3F1-8 | 10.5-kb fragment containing DSE1, RSP5, and NSA1 in YCplac111 | This study |
| pFA499Rc | 4.3-kb fragment containing RSP5 promoter-RSP5 in YCplac33 | This study |
| pFA499Re | 4.3-kb fragment containing RSP5 promoter-RSP5 in YEplac195 | This study |
| pFA18Ac | 4.3-kb fragment containing RSP5 promoter-HPG1-1 in YCplac33 | This study |
| pFA18Ae | 4.3-kb fragment containing RSP5 promoter-HPG1-1 in YEplac195 | This study |
| pFA12Cc | 4.3-kb fragment containing RSP5 promoter-HPG1-2 in YCplac33 | This study |
| pFA12Ce | 4.3-kb fragment containing RSP5 promoter-HPG1-2 in YEplac195 | This study |
| pFA171Ec | 4.3-kb fragment containing RSP5 promoter-HPG1-3 in YCplac33 | This study |
| pFA171Ee | 4.3-kb fragment containing RSP5 promoter-HPG1-3 in YEplac195 | This study |
| pFA29Ec | 4.3-kb fragment containing RSP5 promoter-HPG1-4 in YCplac33 | This study |
| pFA29Ee | 4.3-kb fragment containing RSP5 promoter-HPG1-4 in YEplac195 | This study |
| pFA51Rc | 4.3-kb fragment containing RSP5 promoter-rsp5-1 in YCplac33 | This study |
| pFA51Re | 4.3-kb fragment containing RSP5 promoter-rsp5-1 in YEplac195 | This study |
| pFA51Ri | 4.3-kb fragment containing RSP5 promoter-rsp5-1 in YIplac211 | This study |
| pFA113Mc | 4.3-kb fragment containing RSP5 promoter-mdp1-13 in YCplac33 | This study |
| pFA113Me | 4.3-kb fragment containing RSP5 promoter-mdp1-13 in YEplac195 | This study |
| YEpnpi1ΔC2 | 6.5-kb fragment containing RSP5 promoter-NPI1/RSP5 containing a deletion of codons for the C2 domain in plasmid 2μm | 65 |
| YEpnpi1ΔWWP | 6.5-kb fragment containing RSP5 promoter-NPI1/RSP5 containing a deletion of codons for the WW domain in plasmid 2μm | 65 |
| YEpnpi1ΔCys | 6.5-kb fragment containing RSP5 promoter-NPI1/RSP5 containing a deletion of codons for the active-site cysteine in plasmid 2μm | 65 |
| pAS55c | TAT2 promoter-2HA-TAT2 in YCplac33 | 11 |
| pTB306c | pAS55c containing a deletion of codons 2 through 10 of TAT2 | 11 |
| pTB307c | pAS55c containing a deletion of codons 2 through 17 of TAT2 | 11 |
| pTB313c | pAS55c containing a deletion of codons 2 through 20 of TAT2 | 11 |
| pTB288c | pAS55c containing a deletion of codons 2 through 29 of TAT2 | 11 |
| pTB373c | pTB288c with a nucleotide substitution changing lysine codon 31 of TAT2 to an arginine codon | 11 |
| pTB359c | pAS55c containing a deletion of codons 2 through 31 of TAT2 | 11 |
| pTB294c | pAS55c containing a deletion of codons 17 through 31 of TAT2 | 11 |
| pTB355c | pAS55c with nucleotide substitutions changing lysine codons 10, 17, 20, 29, and 31 of TAT2 to arginine codons | 11 |
| pBUL1e | 6.5-kb fragment containing BUL1 promoter-BUL1 in YEplac195 | 70, 71 |
| pBUL2e | 6.5-kb fragment containing BUL2 promoter-BUL2 in YEplac195 | 70, 71 |
| YEp105LEU | c-Myc-tagged ubiquitin regulated by the copper-inducible CUP1 promoter in YEp105 in which TRP1 was replaced with LEU2 | 22 |