TABLE 3.

Oligonucleotide primers used in this study

Primer	Deletion and/or HA tag	Sequencea
hisBUL1a	BUL1	5′-ATGGCCAAAGATTTGAACGATTCGGGGTTTCCACCGAAGAGGAAGCCTTTGCTGCGTCCT-GGTGAGCGCTAGGAGTCACT-3′
hisBUL1b	BUL1	5′-TTATTTTGTCACTTGCCTAACAGAAATAGGGATATCAATCTTCGCTACGCCAAGATGGTT-ACACCGCATATGATCCGTCG-3′
hisBUL2a	BUL2	5′-ATGACTTTTACATTCTCCACTTCATCAAGGAAAAATGGGAGACCTCCTTTAAAATCAGTT-GGTGAGCGCTAGGAGTCACT-3′
hisBUL2b	BUL2	5′-CTAAATTTGTAACTTTTTCACATCAACCGGAATATCAACTTTCATAGAACCCAAGTGGTT-ACACCGCATATGATCCGTCG-3′
uraBUL1a	BUL1	5′-ATGGCCAAAGATTTGAACGATTCGGGGTTTCCACCGAAGAGGAAGCCTTTGCTGCGTCCT-AATTCAATTCATCATTTTTT-3′
uraBUL1b	BUL1	5′-TTATTTTGTCACTTGCCTAACAGAAATAGGGATATCAATCTTCGCTACGCCAAGATGGTT-TGGATTAGTCTCATCCTTCA-3′
uraBUL2a	BUL2	5′-ATGACTTTTACATTCTCCACTTCATCAAGGAAAAATGGGAGACCTCCTTTAAAATCAGTT-AATTCAATTCATCATTTTTT-3′
uraBUL2b	BUL2	5′-CTAAATTTGTAACTTTTTCACATCAACCGGAATATCAACTTTCATAGAACCCAAGTGGTT-TGGATTAGTCTCATCCTTCA-3′
leuBUL2a	BUL2	5′-ATGACTTTTACATTCTCCACTTCATCAAGGAAAAATGGGAGACCTCCTTTAAAATCAGTT-ATCAAATTCGATGACTGGAA-3′
leuBUL2b	BUL2	5′-CTAAATTTGTAACTTTTTCACATCAACCGGAATATCAACTTTCATAGAACCCAAGTGGTT-AACTGGCAAACCGAGGAACT-3′
uraTAT1a	TAT1	5′-ATGGACGATAGTGTCAGTTTCATTGCCAAAGAGGCCAGTCCAGCACAATATTCGCACAGT-AATTCAATTCATCATTTTTT-3′
uraTAT1b	TAT1	5′-TTAGCACCAGAAATTGGTCATCCTCTTAAAAAACTTTCTTGAACGCGAGCTGTTTGGATT-TGGATTAGTCTCATCCTTCA-3′
uraTAT2a	TAT2	5′-ATGACCGAAGACTTTATTTCTTCTGTCAAGCGTTCAAATGAGGAGCTGAAGGAGCGAAAAAATTCAATTCATCATTTTTT-3′
uraTAT2b	TAT2	5′-TTAACACCAGAAATGGAACTGTCTCACGTACCATGGACGGGAATCTAGGTACATTTTCTTTGGATTAGTCTCATCCTTCA-3′
hisTAT2a	TAT2	5′-ATGACCGAAGACTTTATTTCTTCTGTCAAGCGTTCAAATGAGGAGCTGAAGGAGCGAAAA-GGTGAGCGCTAGGAGTCACT-3′
hisTAT2b	TAT2	5′-TTAACACCAGAAATGGAACTGTCTCACGTACCATGGACGGGAATCTAGGTACATTTTCTT-ACACCGCATATGATCCGTCG-3′
uraGAP1a	GAP1	5′-ATGAGTAATACTTCTTCGTACGAGAAGAATAATCCAGATAATCTGAAACACAATGGTATT-AATTCAATTCATCATTTTTT-3′
uraGAP1b	GAP1	5′-TTAACACCAGAAATTCCAGATTCTATACCATCTTGGCTTTGTGGCCATAATTGCCTTTTC-TGGATTAGTCTCATCCTTCA-3′
uraDOA4a	DOA4	5′-ATGGAGCAGAATATTATTAGTACCATAAGGGATGAGTGTATTCGTCACCGGTCGAAGTAC-TTTCAATTCAATTCATCATTTTTT-3′
uraDOA4b	DOA4	5′-TCAAACACCGTAGACGCGGTGATAAAACAAAACGTATGCATTAGAGTTAATTGCATCGGC-CAATTGGATTAGTCTCATCCTTCA-3′
uraVPS27a	VPS27	5′-ATGTCCGTTAGCACGCCAAGTGAGTTAGACGCATTAATAGAACAAGCCACTAGTGAGAGCAATTCAATTCATCATTTTTT-3′
uraVPS27b	VPS27	5′-TTAAAGCTCTATTAGCAGTTCCTCTTGAGGACTAGGCGGCCTTTCCTCTTTGATCGGAATTGGATTAGTCTCATCCTTCA-3′
uraVPS45a	VPS45	5′-ATGAACCTTTTTGATGTGGCTGACTTTTATATAAACAAAATTGTGACTTCCCAATCGAAAAATTCAATTCATCATTTTTT-3′
uraVPS45b	VPS45	5′-TTATTTTGCAGATCTAATAGAATCCATATATTCTTTAGTTGAAAGTATAGAGGTGCCTCCTGGATTAGTCTCATCCTTCA-3′
uraPEP12a	PEP12	5′-ATGTCGGAAGACGAATTTTTTGGTGGTGATAATGAAGCCGTTTGGAACGGTTCCAGATTCAATTCAATTCATCATTTTTT-3′
uraPEP12b	PEP12	5′-TTACAATTTCATAATGAGAAAAATAAAAAGAAGCATTACGAGAAGCACAATCAACAAATATGGATTAGTCTCATCCTTCA-3′
rsp5-1a	rsp5-1	5′-AGAGCCCGTTCATTGCAGTTC-3′
rsp5-1b	rsp5-1	5′-GAACTGCAATGAACGGGCTCT-3′
mdp1-13a	mdp1-13	5′-GATTATCGTGATTACCAAGAG-3′
mdp1-13b	mdp1-13	5′-CTCTTGGTAATCACGATAATC-3′
5′-3HA-TAT1	3HA-TAT1	5′-GCTCTAGATGCATAGTCCGGGACGTCATAGGGATAGCCCGCATAGTCAGGAACATCGTATGGGTAGCCAGCGTAGTCTGGGACGTCGTATGGGTACATTTTTACGCCTTTTTATCGAGAAA-3′
3′-3HA-TAT1	3HA-TAT1	5′-CGTCTAGAGACGATAGTGTCAGTTTCATTGCC-3′
5′-3HA-TAT2	3HA-TAT2	5′-CGTCTAGATGCATAGTCCGGGACGTCATAGGGATAGCCCGCATAGTCAGGAACATCGTATGGGTAGCCCGCATAGTCAGGAACATCGTATGGGTACATATGAGAGTGTGTTGCGTAATTT-3′
3′-3HA-TAT2	3HA-TAT2	5′-CGTCTAGAACCGAAGACTTTATTTCTTCTGTC-3′

^aBase substitutions are boldfaced.