TABLE 5.
ΔV ≠ of tryptophan uptake through Tat1 and/or Tat2
| Genotype (permease[s]) | Rate constant (pmol of tryptophan 107 cells−1 min−1)a at the following pressure:
|
ΔV≠ (ml/mol)a of overall process of tryptophan uptake | ||
|---|---|---|---|---|
| 0.1 MPa | 25 MPa | 50 MPa | ||
| Wild type (Tat1 and Tat2) | 3.75 ± 0.69 | 2.14 ± 0.69 | 0.65 ± 0.35 | 91.7 ± 17.3 |
| tat1Δ (Tat2) | 2.40 ± 0.56 | 1.57 ± 0.45 | 0.89 ± 0.34 | 50.8 ± 13.1 |
| tat2Δ (Tat1) | 3.76 ± 0.62 | 2.17 ± 0.43 | 0.65 ± 0.28 | 89.3 ± 17.1 |
Data are means ± standard deviations from 6 (wild type), 5 (tat1Δ), or 11 (tat2Δ) experiments. SD medium rather than SC medium was used to determine rate constants.