FIG. 5.

Nucleosome redistribution driven by ISWI protein. (A) Redistribution of nucleosomes assembled onto the DNA fragment 105A18 directed by ISWI (lanes 2 to 6) is compared to thermal redistribution (lanes 8 to 12). Nucleosomes were incubated with 0.4 pmol of ISWI for 4, 8, 16, 32, and 64 min (lanes 2 to 6). For comparison, nucleosomes were incubated at 47°C for the same times (lanes 8 to 12). (B) Native gel shift assay of the same samples as in panel A. (C) Nucleosome redistribution by thermal incubation and ISWI on the fragment 140A0 using site-directed mapping. Lanes 1 and 4 show that nucleosomes are initially located at the terminal position +70 on this DNA fragment, but following thermal incubation at 47°C (lane 2) or treatment with 0.9 pmol of ISWI (lane 4) for 60 min, nucleosomes are relocated predominantly to the −25 location. (D) Native gel electrophoresis of the samples in panel C.