TABLE 3.
Effects of elongation defects on lacZ-reporter activities
| Strain | Relative β-galactosidase activity witha:
|
||
|---|---|---|---|
| FKS1-lacZ | ADH2-lacZ | GAL1-lacZ | |
| WT | 100 | 100 | 100 |
| hpr1 | 2.3 | 1.6 | 2.2 |
| rpb2-4 | 20 | 12 | 5.5 |
| rpb2-10 | 24 | 13 | 15 |
| rpb2-7 | ND | 290 | 56 |
| spt5-4 | 23 | 2.9 | 4.1 |
| spt5-8 | 65 | 35 | 56 |
| spt5-242 | ND | 59 | 51 |
| spt4 | 1.5 | 4.9 | 0.50 |
a
β-Galactosidase activities were determined for each allele and its corresponding isogenic wild-type parent containing the lacZ reporter as indicated. In order to ease comparison between strains, all wild-type parent values were set to 100 for each reporter. FKS1-lacZ was assayed on medium containing 4% glucose, ADH2-lacZ was assayed on 2% ethanol-2% glycerol, and GAL1-lacZ was assayed on 2% galactose-2% raffinose. All values represent the averages of the results for five separate transformants. The SEMs were less than 20%. ND, no data.