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Concentration dependence of ligand binding and transport by site-directed substitution mutants. (a) Ferric enterobactin binding; (b) ferric enterobactin transport; (c) colicin B binding; and (d) colicin D binding. ○, ++; □, D; ▵, ABC; •, BD; ▪, ABD; and ▴, BCD. Data points are the mean values of at least two experiments.
