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. 2003 Nov;23(21):7600–7610. doi: 10.1128/MCB.23.21.7600-7610.2003

FIG. 3.

FIG. 3.

XIAP and cIAP-1 are overexpressed in SCLC cells and are up-regulated in response to FGF-2 treatment. (A, B, and C) H510 and H69 cells, incubated with or without 25 μM PD098059, were treated with FGF-2 for the times indicated. Cell lysates were analyzed by SDS-PAGE and Western blotting for XIAP and cIAP-1 expression levels. Lamin B immunodetection was used as a protein loading control. (A and C) Optical densitometry was performed on ECL-revealed bands. (D) Equal protein amounts from type II pneumocytes from two independent donors (lanes 1 and 2) and eight SCLC cell lines (lanes 3 to 10) were analyzed by SDS-PAGE and Western blotting for XIAP, cIAP-1, and actin expression levels. Lane 3, HC33; lane 4, H69; lane 5, H209; lane 6, H510; lane 7, H524; lane 8, H1045; lane 9, H1622; lane 10, H2171. (E) Maximal rescue of H510 cells by FGF-2 requires 4 h of preincubation prior to etoposide treatment. H510 cells were incubated with FGF-2 for the times indicated prior to etoposide (VP-16) addition. Cells were then left to incubate for 4 days, and cell survival was determined by cell counting. (F) H510 cells were treated for 4 h with FGF-2 prior to incubation with or without VP-16 for an additional 6 h. Samples were analyzed by SDS-PAGE and Western blotting for XIAP levels. Results shown are representative of three independent experiments (A through D and F). For panel E, each experiment was performed in quadruplicate.