TABLE 3.
Calculation of total HDAC and HAT expression levels in HA-P300-expressing HeLa cells
| Protein | Populationa | No. of cells | Mean TIb (106) | SEM (104) | P valuec |
|---|---|---|---|---|---|
| HDAC-1 | Untransfected | 255 | 2.50 | 9.13 | 0.15 |
| Transfected | 63 | 2.79 | 14.4 | ||
| HDAC-2 | Untransfected | 232 | 1.92 | 6.93 | 0.15 |
| Transfected | 40 | 2.18 | 15.8 | ||
| HDAC-3 | Untransfected | 202 | 2.43 | 8.44 | 0.18 |
| Transfected | 45 | 2.68 | 15.1 | ||
| HDAC-4 | Untransfected | 234 | 3.21 | 10.7 | 0.15 |
| Transfected | 64 | 3.53 | 18.4 | ||
| CBP | Untransfected | 180 | 2.20 | 13.8 | 0.21 |
| Transfected | 47 | 2.57 | 23.2 | ||
| PCAF | Untransfected | 328 | 2.03 | 6.35 | 0.15 |
| Transfected | 47 | 2.28 | 17.6 | ||
| TAFII250 | Untransfected | 239 | 2.11 | 7.97 | 0.44 |
| Transfected | 68 | 2.23 | 14.5 | ||
| MYSTd | Untransfected | 338 | 2.17 | 6.94 | 0.13 |
| Transfected | 52 | 2.46 | 14.8 |
a
Either untransfected control cells or cells transfected with HA-P300.
b
Calculated by averaging the total signal intensity for each nucleus in a population.
c
Unpaired t tests (two tailed) were performed to identify statistical differences between the mean TI values for the transfected versus untransfected populations. A P value of <0.05 is considered statistically significant.
d
The MYST antibody collectively recognizes six members of the MYST HAT family including ESA1, TIP60, MOZ, MOF, SAS2, and SAS3/YBF.