TABLE 5.

Calculations for the difference in mean N-Ac intensities for both transfected and untransfected GFP-CBP, HA-P300, and ASF-GFP populations

Transfected protein	Antibody	Histone	Cells transfected	No. of cells	Mean N-Ac intensitya	SD	P
GFP-CBP	H2B	H2B	+	56	0.7253	0.5518	<0.0001
			−	1,110	0.4779	0.4660
	Ac3	H3	+	58	0.8406	0.7604	<0.0001
			−	1,111	0.3128	0.2452
	K9	H3	+	55	0.4212	0.3041	0.1159
			−	657	0.3504	0.3218
	K14	H3	+	54	1.3152	0.5448	<0.0001
			−	174	0.7429	0.3138
	AcH4	H4	+	58	1.3168	0.8019	<0.0001
			−	714	0.6266	0.4563
	K5	H4	+	64	0.8724	0.7799	<0.0001
			−	680	0.2260	0.1762
	K8	H4	+	49	1.6284	1.1293	0.5633
			−	219	1.7391	1.2277
	K12	H4	+	52	1.4327	0.7891	0.0006
			−	548	1.0440	0.7728
	K16	H4	+	58	0.8548	0.9250	0.3053
			−	1,406	0.7436	0.8044
HA-P300	H2B	H2B	+	58	1.7423	1.4153	0.0032
			−	211	1.2667	0.9650
	K9	H3	+	58	0.6341	0.4576	0.0612
			−	327	0.5390	0.3342
	K14	H3	+	56	1.4416	0.7895	<0.0001
			−	302	1.0685	0.5528
	AcH4	H4	+	52	0.3951	0.3808	0.0271
			−	210	0.2937	0.2697
	K5	H4	+	49	1.7152	1.2147	<0.0001
			−	273	0.9958	0.9285
	K8	H4	+	54	1.3439	0.8745	<0.0001
			−	247	0.6052	0.2436
	K12	H4	+	49	2.1554	1.3265	0.4543
			−	221	2.3570	1.7754
	K16	H4	+	57	2.3785	1.7211	0.1913
			−	242	2.7992	2.2755
ASF-GFP	H2B	H2B	+	51	1.7145	0.7998	0.0527
			−	274	1.4940	0.7327
	Ac3	H3	+	55	1.3870	0.7336	0.2753
			−	222	1.2790	1.3870
	K9	H3	+	54	1.1687	0.7666	0.1943
			−	224	1.0369	0.6430
	K14	H3	+	55	1.0264	0.4807	0.5536
			−	259	1.0722	0.5293
	K5	H4	+	59	1.6588	0.9061	0.2021
			−	220	1.5051	0.7956
	K8	H4	+	55	1.0354	0.6548	0.1778
			−	323	0.9260	0.5370
	K12	H4	+	48	1.9610	1.0373	0.1747
			−	309	1.7756	0.8518
	K16	H4	+	57	0.5930	0.4817	0.6581
			−	311	0.6239	0.4845

^aCalculated for either the untransfected or the transfected cell population as the mean ratio of Cy3 intensity to DAPI intensity.