FIG. 8.

NS3694 inhibits TNF-induced DEVDase activity but not TNF-induced cytotoxicity in WEHI-S cells. WEHI-S cells were left untreated (UN) or pretreated with indicated concentrations of zVAD-fmk (zVAD) and NS3694 for 1 h before treatment with 0.1 (A) or 1 (B) ng of TNF per ml. (A) The survival of the cells was analyzed by the MTT assay after 14-h treatment. The values are means ± standard deviations (error bars) for three samples in a representative experiment. (B) The cells were lysed after a 5-h treatment, and the DEVDase activity in lysates was assessed by spectrofluorometric quantification of DEVD-AFC cleavage and presented as picomoles per minute. The means ± standard deviations (error bars) for three samples in a representative experiment are shown. The experiment was repeated twice with similar results.