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. 1997 Apr 29;94(9):4582–4586. doi: 10.1073/pnas.94.9.4582

Figure 1.

Figure 1

Time course of ectopic E74 induction. (A) Both w and w; P[hs-E74A] animals were synchronized at puparium formation and aged 4 hr. These prepupae were then heat-shocked at 35°C for the number of minutes indicated. RNA was extracted from whole animals, and the endogenous and heat-induced E74A and E74B mRNAs were detected by Northern blot hybridization as described (31). RNA was also extracted from non-heat-induced w; P[hs-E74A] 0-hr prepupae (0 hr pp) as a positive control for normal levels of endogenous E74A mRNA, and from non-heat-induced w; P[hs-E74A] 5-hr prepupae (5 hr pp) as a negative control for E74A expression. (B) Both w and w; P[hs-E74B] animals were synchronized at puparium formation and heat-shocked at 35°C for the number of minutes indicated. RNA was extracted from whole animals, and the endogenous and heat-induced E74A and E74B mRNAs detected by Northern blot hybridization. RNA was also extracted from non-heat-induced w; P[hs-E74B] −8-hr larvae (−8 hr pp) as a positive control for normal levels of endogenous E74B mRNA, and from non-heat-induced w; P[hs-E74B] 1.5-hr prepupae (1.5 hr pp) as a negative control for E74B expression.