Figure 3.

Ectopic E74B represses late gene transcription. The Northern blots of w, w; P[hs-E74A], and w; P[hs-E74B] RNA described in Fig. 2 were hybridized with a radiolabeled probe to detect (A) DHR3 mRNA or (B) L71–1, L71–6, and 4F late mRNAs. A different time point was used to detect E78B mRNA (A), where w and w; P[hs-E74A] late third instar larvae were subjected to heat shock and recovery approximately 8 hr before puparium formation. This time corresponds to the initial induction of E78B, which occurs earlier than that of DHR3. The slightly reduced levels of L71–6 and 4F mRNA in control prepupae reflect standard experimental variation for this stage in development.