Figure 4.

Identification of catfish VH family usage in δ transcripts by RT-PCR. (a) RNA from freshly isolated PBLs was subjected to RT-PCR using forward primers specific for each of the seven described catfish VH families with a reverse primer at the 3′ end of the δ1 exon. The amplified products were electrophoresed on a 1% agarose gel and stained with ethidium bromide. The sizes of two HaeIII φX/174 DNA markers, 1078 and 872 (bp) are indicated by arrows. (b) Cμ1 sequences in the VH-δ1 PCR products were detected by blot hybridization analysis with a Cμ1 probe.