Figure 1.

NK line lysis of B cell targets transfected or loaded exogenously with GAD. (A) The group 2-specific NK lines were prepared from donor DP and incubated with [³⁵S]methionine-labeled target cells for 5 hr at various E:T ratios. E:T ratios of 10:1 and 5:1 are shown. The figure shows one out of three representative experiments. (B and C) HLA-C expression on Priess and the transfectant Priess/pIiGAD. Priess cells were stained with the pan-class I mAb W6/32 antibody (boldface lines) or with the HLA-C specific mAb L31 (dotted line). Controls were the same cells stained with isotype matched IgG (filled histogram). All cells were then counterstained with goat anti-mouse IgG bound to fluorescein isothiocyanate (FITC). The figures show one of four representative experiments.