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Binding of various peptides to HLA-Cw7 and HLA-Cw6. RMA-S cells transfected with HLA-Cw7 (A) or HLA-Cw6 (B) were cultured overnight at 37°C with (boldface line) or without (lightface line) added peptide at a final concentration of 10 μM. Cells were stained with mAb W6/32 and analyzed by fluorescence-activated cell sorting (FACS). HLA-C-specific fluorescence shift is indicated by increased intensity of staining compared with no added peptide control.
