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Binding of P8 Lys and Ala substituted peptides to HLA-Cw7. RMA-S cells transfected with HLA-Cw7 were cultured overnight with (boldface line) or without (lightface line) the added peptide at a final concentration of 10 μM. Cells were stained with mAb W6/32 and analyzed by fluorescence-activated cell sorting (FACS).
