Fig. 1.

Regional differences in gene expression discriminate between STG and remaining CTX. RNA from midgestation human STG and CTX (average of 18.6 weeks old) were labeled with Cy3 and Cy5, respectively, and pairs of samples hybridized against Agilent G4110A arrays. After normalization of expression levels with Agilent Feature Extraction software (Ver. 7.5.1), single-channel values were analyzed for >1.5-fold differences using the Limma package from Bioconductor/R. An unsupervised clustering algorithm, based on Euclidian Distance, was then used to calculate intersample relationships using expression values for these DE genes (n = 343; 44 up- and 299 down-regulated). In contrast to regional clustering, no effect was observed for sex, side of origin (Left or Right), or developmental age (data not shown).