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. 2007 Oct 29;104(45):17765–17770. doi: 10.1073/pnas.0708767104

Fig. 3.

Fig. 3.

B7-DC may be the main ligand responsible for the concentration of PD-1 at the immunological synapse. (A) Splenic DCs from WT or B7-DC−/− B6 mice were purified and cultured as in Fig. 2. The WT DCs are in red (LPS) and blue (IL-4), and the B7-DC−/− cells are in green (LPS) and orange (IL-4). (B) OT-II T cells were infected with CD28-YFP or PD-1-YFP. Conjugates with concentrated PKC θ (blue) at the synapse were scored for the localization of either costimulatory molecule (yellow) to the synapse. CD11c is in red. (Top) PD-1 at the synapse between OT-II T cells and WT DCs. (Middle and Bottom) Conjugates between OT-II T cells and B7-DC−/− DCs. A representative cell with concentrated PD-1 is in Middle, and a cell with uniform distribution of PD-1 is in Bottom. (C) The statistics from three experiments with at least 30 conjugates are shown as mean and SD (error bars). The numbers after the bars are the same as in Fig. 2.