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. 2007 Oct 25;104(45):17692–17697. doi: 10.1073/pnas.0707045104

Fig. 1.

Fig. 1.

The FLI1+12 hematopoietic enhancer targets blood stem cells. (A) The FLI1+12 enhancer directs reporter activity to blood and blood vessels. (i) Schematic diagram of the human FLI1 locus. A fragment of DNA corresponding to the FLI1+12 region was used to generate transgenic mice. (ii–vii) E7.5–E12.5 X-Gal-stained whole-mount WT and L5760 SV/lacZ/FLI1+12 embryos. (ii) E7.5 WT embryo with no staining. (iii) E7.5 transgenic embryo showing staining within the extra-embryonic hematopoietic region. (iv) E9.5 transgenic embryo showing staining of the heart chambers (boxed) and vitelline vessels (arrow). (v) E11.5 transgenic embryo showing staining within the FL (arrow) and blood vessels. (vi) E12.5 transgenic embryo showing staining in the vitelline vessels (arrow). (vii) Magnified view of the boxed area in vi showing FL staining (arrow). (viii–xiii) Paraffin sections of a E11.5 SV/lacZ/FLI1+12 embryo. (viii) Sagittal section of the DA showing staining in the endothelium. (ix) High-power view of the DA showing staining in a hematopoietic cluster (arrow). (x) Section through the FL showing staining in hematopoietic cells. (xi) High-power view of x. (xii) Section through the yolk sac showing staining in the wall of a blood vessel. (xiii) Section through the heart showing endocardial staining. (B) Flow cytometry of FDG treated nontransgenic and transgenic E12.5 FLs from a litter of SV/lacZ/FLI1+12 (L5760) × WT crosses. CD150+/CD48−/CD41− cells are enriched in the population targeted by the transgene. YS, yolk sac.