Fig. 2.

Conserved Ets and Gata TF binding sites in the FLI1+12 enhancer are required for its activity in hematopoietic cells. (A) Nucleotide sequence alignment of the FLI1 +12 enhancer with conserved Gata, Ets, and E-box sites marked in red, blue, and pink, respectively. Nucleotide numbering is from the translation start site. mm, mouse; hs, human; md, opossum. (B) (Left) Reporter constructs of human WT and mutated genomic fragments corresponding to the FLI1+12 enhancer. The conserved Ets, Gata, and E-box sites are represented as circles (dashed margins for partial conservation), numbered E1–8, G1–2, and EB, respectively; they are crossed out where mutated. (Right) Results of stable transfection assays in 416B cells corresponding to each construct. The luciferase activities are given as fold-increase over the activity of the pGL2-promoter vector (SV-luc) alone. (C) Summary of F₀ transgenic embryos generated with various SV/lacZ/mutant FLI1+12 constructs. Representative X-Gal-stained whole-mount E11.5 embryos are also shown. (+) to (++++) indicates from week/rare to strong X-Gal staining.