Table 3.
Predicted frequencies of embryonic lethality (DL) and offspring with reciprocal translocations (HT) in mouse zygotes (1-Cl) zygotes of three maternal genotypes mated to unirradiated (0 Gy) or irradiated (4 Gy) male
| Maternal genotype | Paternal exposure, Gy* | DAPI analysis |
PAINT analysis |
||||||
|---|---|---|---|---|---|---|---|---|---|
| No. of 1-Cl analyzed | 1-Cl with unstable aberrations | Predicted DL† | No. of 1-Cl analyzed‡ | 1-Cl with unstable aberrations | 1-Cl with stable aberrations | Normal zygotes | Predicted HT§ | ||
| C57BL/6J | 0 | 117 | 2 | 1.3 ± 0.7 | 69 | 1 | 1 | 67 | 0 |
| C57BL/6J | 4 | 261 | 51 | 19.5 ± 1.4 | 149 | 22 | 11 | 116 | 8.7 ± 2.9 |
| scid | 0 | 61 | 1 | 1.6 ± 1.1 | 36 | 1 | 0 | 35 | 0 |
| scid | 4 | 71 | 31 | 43.7 ± 8.1 | 42 | 9 | 2 | 31 | 6.1 ± 3.2 |
| Rad54-null | 0 | 175 | 4 | 2.3 ± 0.9 | 103 | 3 | 0 | 100 | 0 |
| Rad54-null | 4 | 155 | 42 | 27.1 ± 2.4 | 76 | 11 | 3 | 62 | 4.6 ± 1.9 |
*Seven days prior to mating; males were B6C3F1 for all the experiments.
†Percentage ± SE observed frequency of dominant lethality: 24.5% (see ref. 29).
‡Cell equivalents. See Materials and Methods.
§1-Cl with reciprocal translocations over the number of viable zygotes (normal plus reciprocal translocations). Percentage ± SE. Observed frequency of translocation carriers: 14.5% (see ref. 31).