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. 2007 Oct 30;104(45):17662–17667. doi: 10.1073/pnas.0700411104

Fig. 4.

Fig. 4.

Association quotients (Q) for cells expressing T cell surface proteins incubated at 37°C with Alexa488- and Alexa647-labeled lectin and Fab fragments. (A) The DO11.10 T cell hybridoma incubated with dually and singly labeled WGA. (B) Truncated CD86 monomer- and CD28 homodimer-expressing DO11.10 T cell hybridomas incubated with Alexa488- and Alexa647-labeled anti-CD86 and anti-CD28 Fab fragments. (C) The DO11.10 T cell hybridoma incubated with Alexa488- and Alexa647-labeled anti-TCRβ and anti-CD3ε Fab fragments in the combinations indicated. The first Fab fragment of each pair shown was labeled with Alexa488, and the second was labeled with Alexa647. The maximum coincidence detection efficiency in these experiments is estimated to be 7%; see text and SI Materials and Methods for details.