Table 1.
Association quotients for T cell surface proteins labeled at 37°C with singly or dually fluorescently labeled WGA, or with pairs of fluorescently labeled Fab fragments, together with the significant event rate (the rate of coincident events above the rate due to random diffusion), the rate of red and blue events, and the numbers of cells and events analyzed
| Association quotient × 103, Q | Coincident event rates, s−1 | Red event rates, s−1 | Blue event rates, s−1 | No. of cells analyzed | No. of events analyzed | No. of detectable molecules in probe area | |
|---|---|---|---|---|---|---|---|
| Single WGA | 3.3 ± 1.5 | 0.88 | 25.0 | 19.5 | 34 | 690,000 | 0.45 |
| Dual WGA | 16.4 ± 2.8 | 1.27 | 19.8 | 20.7 | 18 | 800,000 | 0.41 |
| CD86/CD86 | 8.0 ± 2.6 | 0.46 | 10.6 | 7.1 | 23 | 280,000 | 0.44 |
| CD28/CD28 | 18.3 ± 3.3 | 0.47 | 8.2 | 6.2 | 32 | 250,000 | 0.36 |
| TCRβ/CD3ε | 18.0 ± 2.3 | 0.62 | 10.7 | 10.3 | 170 | 1,900,000 | 0.53 |
| CD3ε/CD3ε | 17.3 ± 2.4 | 0.64 | 11.3 | 9.4 | 59 | 450,000 | 0.52 |
| TCRβ/TCRβ | 7.1 ± 1.1 | 0.47 | 10.8 | 9.8 | 150 | 1,400,000 | 0.52 |
The last column shows the average number of red and blue molecules in the probe area in each experiment.