Table 1.
Association quotient × 103, Q | Coincident event rates, s−1 | Red event rates, s−1 | Blue event rates, s−1 | No. of cells analyzed | No. of events analyzed | No. of detectable molecules in probe area | |
---|---|---|---|---|---|---|---|
Single WGA | 3.3 ± 1.5 | 0.88 | 25.0 | 19.5 | 34 | 690,000 | 0.45 |
Dual WGA | 16.4 ± 2.8 | 1.27 | 19.8 | 20.7 | 18 | 800,000 | 0.41 |
CD86/CD86 | 8.0 ± 2.6 | 0.46 | 10.6 | 7.1 | 23 | 280,000 | 0.44 |
CD28/CD28 | 18.3 ± 3.3 | 0.47 | 8.2 | 6.2 | 32 | 250,000 | 0.36 |
TCRβ/CD3ε | 18.0 ± 2.3 | 0.62 | 10.7 | 10.3 | 170 | 1,900,000 | 0.53 |
CD3ε/CD3ε | 17.3 ± 2.4 | 0.64 | 11.3 | 9.4 | 59 | 450,000 | 0.52 |
TCRβ/TCRβ | 7.1 ± 1.1 | 0.47 | 10.8 | 9.8 | 150 | 1,400,000 | 0.52 |
The last column shows the average number of red and blue molecules in the probe area in each experiment.