Fig. 3.

Association of HA-tagged INAF-PB with TRP channels. (A) Immunolocalization. A representative confocal section of retina, costained with anti-TRP and anti-HA, from adult flies bearing the tagged version of inaF-PB, is shown. To eliminate interference by eye pigment, the transgenic line also carried bw and st mutations. Within the ommatidium shown, signal from INAF-B is detected in rhabdomeres (and not their surrounding cell bodies) and colocalizes with signal from TRP. (B–F) Immunoprecipitation. The top line of the key for each panel shows the antibody used to precipitate dodecyl-β-maltoside extracts of fly heads: anti-HA (H), anti-TRP (T), anti-INAD (D), or a nonspecific control antibody (N). The second line of the key indicates the presence (+) or absence (−) of an HA tag on a transgenic copy of INAF-B. Where appropriate, the key also indicates the wild-type (+) or mutant (−) status of the locus whose genotype varies in the samples of the panel. Immunoprecipitates were electrophoresed and then probed with the antibody indicated at the left of each panel. The lanes with IP status marked (−) contain a sample (≈3%) of material used as input for the corresponding immunoprecipitations.