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(A) Southern blots of reverse transcriptase–PCR of LARD cDNA with primers F LARD Kpn and R LARD Xba probed with 32P-labeled primer F LARD Xba. Lanes: 1, CD4+ cells; 2, CD8+ cells; 3, B cells; 4, PHA-blasted PBL; 5, negative control. (B) Ethidium-stained control PCR with glyceraldehyde-3-phosphate dehydrogenase primers. Lanes: 1 and 7, φX HaeIII markers; 2, negative control; 3, CD4+ cells; 4, CD8+ cells; 5, B cells; 6, PHA-blasted peripheral blood mononuclear cells.
