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The HDAC3 Y(298)H substitution generates a loss-of-function mutant. (A) Enzymatic activities of WT and Y(298)H-mutated HDAC3-FLAG (HDAC3-F) proteins on pepH4-AcK16. (B) Western blot of HDAC3-FLAG immunocomplexes assayed in A by using anti-FLAG (HDAC3-F) or anti-Gal4 (GAL4-DAD) antibodies. Ten, 20, and 40 nM WT and mutant HDAC3 were analyzed.
