Skip to main content
. 2007 Oct 18;104(44):17353–17357. doi: 10.1073/pnas.0708265104

Table 2.

Activity of designed LDHs

Clone No. of mutations Relative LDH activity, (kcat/Km) Average gain per mutation, fold*
Start eMDH R81Q 0 1
Designed eMDH pentamutant† 4 30 2.3
Natural end LDH ca. 220 104 1.04

*x4 = 30; x = 2.3 and x220 = 104; x = 1.04.

†eMDH I12V/R81Q/M85E/G210A/V214I mutant.