Figure 3.

Neonatal tolerization using the CDR-based peptides decrease autoantibody titers. Mice were tolerized and immunized as described. Two months following immunization, mice were bled and their sera subjected to ELISA experiments to test the levels of anti-single-stranded DNA (a), antinuclear antigens (b), and 16/6 Id⁺ (c) specific antibodies. The groups include mice tolerized with peptide pCDR1 and immunized with either peptide pCDR1 (□) or with mAb 5G12 (○), mice tolerized with the nonrelevant peptide p307 and immunized with either peptide pCDR1 (▪), or mAb 5G12 (•) and normal BALB/c mice (∗). Results are expressed as mean OD ± SD.