Table 2.
Binding of the CDR-based peptides to MHC class II on live splenic APC
| Mouse strain | % binding
|
H-2 | mAb used | % inhibition
|
||
|---|---|---|---|---|---|---|
| pCDR1 | pCDR3 | pCDR1 | pCDR3 | |||
| BALB/c | 45 | 41 | d | anti I-Ad (MKD6) | 76.7 | 100 |
| BALB/c | d | anti I-Ab (34-5-3) | 0 | 0 | ||
| SJL | 42 | 43 | s | anti I-As (10.3.6.2) | 100 | 92.8 |
| SJL | s | anti I-Ad (MKD6) | 0 | 0 | ||
| C3H.SW | 42 | 53 | b | anti I-Ab (34-5-3) | 60 | 84.4 |
| C3H.SW | b | anti I-Ad (MKD6) | 0 | 25 | ||
| C57BL/6 | 9 | 8.5 | b | |||
| C57BL/6 | b | |||||
Splenic adherent cells were incubated for 16 hr at 37°C with the biotinylated peptides in the presence or absence of anti-Ia mAb, stained, and analyzed thereafter as described.