Table 2.
Organism | Number of gel discs per tube | PEG containing gel |
Lactose containing gel |
||||
---|---|---|---|---|---|---|---|
Bacterial concentration at time (CFU/ml) |
Bacterial concentration at time (CFU/ml) |
||||||
0 h | 24 h | 48 h | 0 h | 24 h | 24 h | ||
S. aureus | 1 Disc | 2.5 × 105 | 1.86 × 103 | 1.72 × 103 | 2.5 × 105 | 2.18 × 103 | 2.06 × 103 |
2 Discs | 1.05 × 103 | 9.06 × 102 | 1.96 × 103 | 1.55 × 103 | |||
3 Discs | 4.80 × 102 | 4.25 × 102 | 1.01 × 103 | 9.05 × 102 | |||
S. pyogenes | 1 Disc | 2.27 × 105 | 1.49 × 103 | 1.19 × 103 | 2.27 × 105 | 1.79 × 103 | 1.48 × 103 |
2 Discs | 1.01 × 103 | 6.05 × 102 | 1.19 × 103 | 7.95 × 102 | |||
3 Discs | 2.55 × 102 | 1.55 × 102 | 6.00 × 102 | 3.65 × 102 | |||
Diphtheroid | 1 Disc | 2.33 × 105 | 5.80 × 102 | 4.25 × 102 | 2.33 × 105 | 8.45 × 102 | 6.85 × 102 |
2 Discs | 2.10 × 102 | 90 | 5.35 × 102 | 3.90 × 102 | |||
3 Discs | 9 | 15 | 3.10 × 102 | 2.35 × 102 | |||
P. aeruginosa | 1 Disc | 2.28 × 105 | 5.95 × 102 | 1.80 × 102 | 2.28 × 105 | 8.20 × 102 | 3.85 × 102 |
2 Discs | 0 | — | 0 | — | |||
3 Discs | 0 | — | 0 | — | |||
E. coli | 1 Disc | 2.32 × 105 | 1.36 × 103 | 8.95 × 102 | 2.28 × 105 | 1.98 × 103 | 1.52 × 103 |
2 Discs | 0 | — | 0 | — | |||
3 Discs | 0 | — | 0 | — | |||
A. baumanii | 1 Disc | 2.35 × 105 | 9.15 × 102 | 7.85 × 102 | 2.35 × 105 | 1.13 × 103 | 9.80 × 102 |
2 Discs | 5.70 × 102 | 4.45 × 102 | 7.65 × 103 | 6.45 × 102 | |||
3 Discs | 2.55 × 102 | 1.75 × 102 | 5.05 × 102 | 3.75 × 102 | |||
C. perfringens | 1 Disc | 2.38 × 105 | 1.02 × 103 | 2.38 × 105 | 1.39 × 103 | ||
2 Discs | 7.10 × 102 | 9.06 × 102 | |||||
3 Discs | 3.85 × 102 | 6.05 × 102 | |||||
C. albicans | 1 Disc | 2.33 × 105 | 5.80 × 102 | 4.40 × 102 | 2.33 × 105 | 8.30 × 102 | 7.30 × 102 |
2 Discs | 3.00 × 102 | 2.00 × 102 | 4.65 × 102 | 3.85 × 102 | |||
3 Discs | 1.55 × 102 | 35 | 2.30 × 102 | 1.70 × 102 |
Target microorganisms were seeded at 105 CFU/ml. Parallel tubes were seeded with one to three gels. At 24 and 48 h the CFU/ml were determined. Anaerobic C. perfringens was counted only at 48 h considering their normal growth patterns. Shown are the bacterial counts in CFU/ml. Placement of a control tube having microorganisms at 105 at 0 h gave non log growth counts at 24 and 48 h for aerobes and 48 h for anaerobes (data not shown). The composition of both gels are the same as described in earlier table.